Figure 8. Rac1G12V knock-in fully rescues the Mst1/2-deficient phenotype.
(a) Mst1fl/flMst2fl/fl (WT), Mst1fl/flMst2fl/flLyz2-Cre (cDKO) and cDKO-Rac1G12V BMDMs were infected with GFP-E. coli for the indicated times and the GFP-E. coli was quantified by fluorescence microscopy. Data were assessed with Student's t test and are represented as mean ± s.d. n=5. * p<0.05, ** p<0.01 in a comparison of cDKO and cDKO-Rac1G12V groups.
(b) Mortality of WT (n=11), Rac1G12V (n=10), cDKO (n=10) or cDKO-Rac1G12V (n=11) mice subjected to sublethal CLP. The Mantel–Cox test, p=0.046 compared between cDKO and cDKO-Rac1G12V groups.
Data are from one experiment representative of three independent experiments with similar results (a, b).