Figure 4.

Metabolic Effect of Repopulation

(A) Serum bilirubin levels in the four Gunn rat iHeps (black lines) at the indicated time points after transplantation are shown as percent of pretransplant levels (means ± SD of three determinations for each time point). Dashed black line, untreated age-matched controls (means ± SD of 6 rats); dashed blue line, bilirubin levels in the sham-treated controls; red lines, bilirubin levels after transplantation of primary human hepatocytes; Dotted line, upper limit of serum bilirubin levels in congeneic normal Wistar-RHA rats.

(B) Serum bilirubin levels (mean ± SD) at indicated intervals after transplantation. Open bar, untreated control (n = 6 rats); light blue, sham-operated control (n = 4 rats); black, iHep transplant recipients (n = 4 rats); red, recipients of primary human hepatocytes (n = 2 rats). ^∗p < 0.02.

(C–E) High-pressure liquid chromatographic analysis of bilirubin species excreted in the bile of a control Gunn rat, a rat receiving iPSC-derived hepatocytes, and a congeneic normal Wistar RHA rat. sf, solvent front. The absorbance unit scale in (E) is different from that in (F and G).

See also Figure S3.