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. 2015 Jun 4;5(1):111–124. doi: 10.1016/j.stemcr.2015.05.007

Figure 3.

Figure 3

Blimp-1 Represses Downstream Targets of Pluripotency Network Genes

(A) Gene expression of the germ cell markers is shown. Expression in E11.5 PGCs was compared with that in ESCs (TT2). Relative gene expression is shown (mean ± SD; independent experiments, n = 3). Red bars indicate the expression level in ESCs (set at 1).

(B) Gene expression of germ cell markers in ESCs. The expression in overexpressing ESCs was compared with that in parental ESCs. Relative gene expression is shown (mean ± SD; independent experiments, n = 3). Red bars indicate the expression level in parental ESCs (set at 1). In the case of Blimp-1, expression in ESCs transfected with Blimp-1-IRES-AcGFP was compared with that in ESCs transfected with IRES-AcGFP. Red bars indicate the expression level in ESCs transfected with IRES-AcGFP (set at 1).

(C) GSEA of downstream targets of the pluripotency network from Kim et al. (2008). ESCs overexpressing each germ cell gene were compared with parental ESCs. In the case of Blimp-1, ESCs were transfected with pEF1a-IRES-AcGFP or EF1a-Blimp-1-IRES-AcGFP, and then AcGFP-positive cells were sorted and compared.