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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Hepatology. 2015 Jul 22;62(5):1606–1618. doi: 10.1002/hep.27907

Figure 3. Liver specific NCoR1 deletion in ad libitum fed mice and NCoR1 deletion in MEFs results in a robust induction of lipogenic, ketogenic and OxPhos genes.

Figure 3

(A–C) Gene Set Enrichment Analysis of gene expression profiles in livers of chow fed NCoR1hep−/− mice at 25 weeks of age (n=4/group). The gene set encompassing lipid metabolism has a nominal p = 0.03 and False discovery rate (FDR) q = 0.052 (A), PPARα has nominal p < 0.001 and FDR q = 0.024 (B), and the ERRα has nominal p = 0.028 and FDR q = 0.056 (C). (D and E) qRT-PCR analysis of mRNA expression in the livers of male chow fed mice at 25 weeks of age. (n=7/group). mRNA expression for the indicated genes involved in NADPH synthesis, lipid synthesis, lipid sequestration (D) and OxPhos (E). (F) Western blot analysis showing Atp5a, Uqcrc2, Mtco1, Sdhb and Ndufb8 levels in NCoR1hep+/+ and NCoR1hep−/− livers of chow fed 25-wk old male NCoR1hep+/+ and NCoR1hep−/− mice. (G) BN-PAGE of OXPHOS complexes in mitochondria isolated from NCoR1hep+/+ and NCoR1hep−/− livers.