Table 1. Transport of Na+ and L-Asp in GltPh.
Mutant | Km for Na+ (mM) | Hill coefficient for Na+ uptake | Km for Asp (nM) |
---|---|---|---|
Wild type a | 8.4 ± 1.3 | 2.5 ± 0.3 | 119 ± 18 |
T308A | 47.6 ± 6.3 | 1.1 ± 0.1 | 220 ± 24 |
T308S | 9.2 ± 2.3 | 2.3 ± 0.7 | 134 ± 27 |
T308V | 18.4 ± 6.3 | 1.7 ± 0.3 | 183 ± 26 |
T308W | N.D. | N.D. | N.D. |
aErrors are represented as s.e.m.