Fig 2. In vivo quantification of inflammation load in the spinal cord and vertebral bone marrow in EAE rats.

¹⁹F signal in the spinal cord and vertebral bone marrow is significantly increased in EAE rats compared to wild-type rats (black vs. grey bars; p < 0.05 indicated by *). ¹⁹F signal in the spinal cord and vertebral bone marrow of EAE rats is significantly decreased by prophylactic treatment with cyclophosphamide (black vs. white bars; p < 0.05 indicated by **). The apparent number of fluorine atoms was measured by integrating the signal in ROIs and normalizing the results to the signal in a reference capillary containing a known concentration of fluorine, as discussed in Methods section.