Table 3.
Summary | Control 0 h | Exposure to Sulfide | |||
---|---|---|---|---|---|
6 h | 24 h | 48 h | |||
Raw data | Total number | 4,089,259 | 3,772,420 | 4,214,487 | 4,218,738 |
Distinct tag number | 223,037 | 194,769 | 183,978 | 196,142 | |
Clean tags | Total number | 3,909,160 | 3,613,737 | 4,057,279 | 4,027,932 |
Distinct tag number | 94,189 | 86,979 | 82,866 | 82,991 | |
All tags mapping to a gene | Total number | 3,381,053 | 2,982,514 | 3,406,833 | 3,372,408 |
Total % of clean tags | 86.49 % | 82.53 % | 83.97 % | 83.73 % | |
Distinct tag number | 60,344 | 49,296 | 49,136 | 47,831 | |
Distinct tag % of clean tags | 64.07 % | 56.68 % | 59.30 % | 57.63 % | |
Unambiguous tags mapping to a gene | Total number | 2,843,143 | 2,484,170 | 2,865,778 | 2,839,135 |
Total % of clean tag | 72.73 % | 68.74 % | 70.63 % | 70.49 % | |
Distinct tag number | 48,405 | 39,557 | 39,685 | 38,365 | |
Distinct tag % of clean tags | 51.39 % | 45.48 % | 47.89 % | 46.23 % | |
All tag-mapped genes | Number | 21,121 | 20,526 | 20,129 | 20,111 |
% of reference genes | 40.54 % | 39.40 % | 38.64 % | 38.61 % | |
Unambiguous tag-mapped genes | Number | 14,722 | 14,264 | 13,937 | 13,832 |
% of reference genes | 28.26 % | 27.38 % | 26.75 % | 26.55 % | |
Unknown tags | Total number | 502,708 | 591,656 | 601,170 | 601,065 |
Total % of clean tags | 12.86 % | 16.37 % | 14.82 % | 14.92 % | |
Distinct tag number | 32,399 | 35,742 | 31,937 | 33,194 | |
Distinct tag % of clean tags | 34.40 % | 41.09 % | 38.54 % | 40.00 % |
The raw data are tags obtained from sequencing without processing. The clean tags are tags that remained after filtering out dirty (low-quality) tags from the raw data. The distinct tags are tags that do not overlap. The unambiguous tags are the clean tags that remained after the removal of tags mapped to reference sequences from multiple genes