Table 4.

DEGs and their associated pathways in U. unicinctus exposed to sulfide

Pathway	U. unicinctus Unigene accession number	Gene annotation	Differential expression multiplication log2 Ratio (TPM time/ TPM control)
			6 h	24 h	48 h
Apoptosis
	Unigene6112_No.2	caspase-3	2.23	2.90	1.85
	CL530.Contig1_No.2	caspase-7	2.16	1.73	1.54
Glycolysis
	CL3519. Contig1_No.2	pyruvate kinase	−0.64	−1.08	−0.85
	Unigene2735_No.2	phosphorylase	−0.43	−1.08	−0.58
	CL1308. Contig1_No.2	phosphofructokinase	−0.70	−0.59	−1.08
	Unigene1913_No.2	ADP-dependent glucokinase	−6.77	−4.93	−6.34
	CL5108.Contig1_No.2	phosphoglycerate kinase	−1.68	−1.61	−1.40
MAPK
	Unigene2294_No.2	cPLA2	3.38	4.17	2.67
	Unigene1096_No.2	RSK2	3.61	2.12	2.40
NF-κB
	Unigene8158_No.2	TLR4	3.09	1.24	1.30
	Unigene15896_No.2	Myd88	3.06	1.95	2.27
	Unigene3552_No.2	NEMO	1.45	0.94	0.61
	Unigene5808_No.2	RIP1	3.68	1.44	1.59
Oxidative phosphorylation
	Unigene1548_No.2	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2-like	−1.76	−1.07	−1.48
	Unigene1556_No.2	NADH-ubiquinone oxidoreductase	−2.01	−2.56	−1.79
	Unigene2512_No.2	NADH dehydrogenase	−8.48	−8.48	−8.48
	Unigene1586_No.2	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9-like	−1.13	−0.75	−0.87
	Unigene2306_No.2	succinate dehydrogenase [ubiquinone] iron-sulfur subunit	−1.24	−0.33	−0.31
	Unigene1680_No.2	ubiquinol-cytochrome c reductase	1.06	0.68	0.44
	Unigene1299_No.2	cytochrome c oxidase subunit I	1.00	1.21	1.43
	Unigene1383_No.2	mitochondrial H+ ATPase a subunit	1.19	0.51	0.47
p53
	CL5080.Contig1_No.2	PIDD	4.68	3.07	3.26
Sulfide oxidation
	Unigene2733_No.2	sulfide-quinine oxidoreductase	0.35	0.47	1.30
	Unigene4395_No.2	sulfur dioxygenase	−0.83	−0.10	−0.20
	Unigene1081_No.2	sulfur transferase	0.13	0.27	0.20