Fig. 1.

Incubation of H. pylori with AGS cells resulted in dephosphorylation of PDK-1 at Ser 241. a After infection with H. pylori strains SS1, 26695, 60190, G27, or SD4, total AGS protein was separated by SDS-PAGE followed by Western blot to detect phopho Ser 241 PDK-1 (upper panel) and total PDK-1 protein (lower panel). b H. pylori, strain 60190 incubated with AGS cells for 24 h and PDK-1 phosphorylation at Ser 241 and total protein levels were quantified. Both phospho Ser 241 and total protein levels were normalized to the housekeeping protein Hsp27. The amount of phosphorylation or total PDK-1 protein is expressed as a ratio of control protein, i.e. (phospho protein/Hsp27)/(total protein/Hsp27). Data are representative of at least 5 different experiments and presented as a percent of control and indicate the relative amount of phosphorylation or protein remaining in the system