Fig. 2.

H. pylori associated dephosphorylation of PDK-1 alters the phosphorylation stability of Akt. a After infection with H. pylori strains SS1, 26695, 60190, G27, or SD4, total AGS protein was separated by SDS-PAGE followed by Western blot to detect phopho Ser 473 Akt (upper panel), total Akt (middle panel), or Hsp27 (bottom panel). b H. pylori, strain 60190 was incubated with AGS cells for 24 h and Akt phosphorylation at Ser 473 and total protein levels were quantified. Both phospho Ser 473 and total protein levels were normalized to the housekeeping protein Hsp27 and expressed as a ratio of control protein, i.e. (phospho protein/Hsp27)/(total protein/Hsp27). Data are representative of at least 3 different experiments and presented as a percent of control and indicate the relative amount of phosphorylation or protein remaining in the system