Fig. 3.

PDK-1 dephosphorylation in the presence of H. pylori is indirect. a Representative Western blot of phospho Ser 241 PDK-1 and total PDK-1 protein following incubation of AGS cells with live (lane 2) or heat-killed H. pylori (lane 3). Total PDK-1 protein ran as two distinct species, one at the expected molecular weight (arrow) and at a faster migrating band (*) that was likely proteolytically cleaved. b Incubation of whole H. pylori lysates with purified recombinant His₆-PDK-1. c Incubation of AGS cells with H. pylori in the absence or presence of Src inhibitor, PP2