DNA content, GAG content, and collagen content found in engineered constructs (column 1) and cumulative release to the culture medium (column 2) for different mixing intensities. For column 1 a “Δ” denotes a significant difference with respect to 0.29 weeks cultures unless otherwise specified within a given mixing intensity, a “” denotes significant differences compared to all other 0.29 weeks values of different mixing intensities unless otherwise specified, a “” denotes a difference compares to all other 2 weeks values, and a “” denotes a difference compared to all other 6 weeks values. In column 2 a “Δ” denotes a significant difference between media values for that given time point, and the remainder denote differences between respective time points for a given mixing intensity: “” = static, “” = Re 0.5, “” = Re 2.9, “” = Re 5.8, “” = 10.2, and “” = Re 21.8. For symbols not accompanied by bars signifies a significantly higher concentration than all other time points. Data presented as mean ±SD and P < 0.05.