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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Inflamm Bowel Dis. 2014 Feb;20(2):363–377. doi: 10.1097/01.MIB.0000440612.98950.79

Figure 5. 10% zinc formalin fixation preserved fluorescence signals from 680 fluorophore conjugated probes, but not signals from 750 fluorophore conjugated probes.

Figure 5

Fresh ileum and colon from both DSS-treated and water control animals were fixed in 10% zinc formalin for overnight and kept in 1XPBS in the dark before re-imaging. Activation pattern of 680 fluorophore conjugated probes was preserved after 10% zinc formalin fixation (A–D), however, Activation of 750 fluorophore conjugated probes were not retained after fixation (E–G). (H) Activation of ProSense 680 is enhanced on fixed versus fresh tissue in intestine tissue from water control animal.

I:ileum; PC: Proximal colon; DC: Distal colon