Figure 7. Liquid diet feeding significantly reduced gastrointestinal auto-fluorescence background during in vivo FMT imaging of mice.

(A) Representative 3D FMT imaging of mouse abdomen from WT, un-diseased control animals that received liquid diet minus or plus MMPSense 680 probe injection or Trylite and fasting but not probe. Images were acquired at excitation of 680 nm. Cube ROIs (in purple color) were placed on abdomen to quantify the absolute fluorescence signal (pmol). (B) Quantification of signal intensity in abdomen demonstrated lower auto-fluorescence with liquid diet feeding (no probe or probes versus Trilyte and fasting). Data are presented as mean±SEM, n=4–6/group. (C) Representative 3D FMT imaging of mouse abdomen from animals that received liquid diet minus or plus ProSense 750 probe injection or Trylite and fasting but no probe (n=4–6). Images were acquired at excitation of 750 nm. (D) Quantification of signal intensity in abdomen demonstrates that ProSense 750 probe yielded background fluorescence signal in probe injected mice but not animals minus probe. (E) 3D FMT imaging at 680nm excitation wavelength in H₂O control and DSS treated animals given no probe injections reveals some background but no difference between H₂O and DSS. (F) 3D FMT imaging at 750 nm excitation wavelength in H₂O control and DSS treated animals given no probe injections reveals no background.