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. 2015 Oct 21;15:97. doi: 10.1186/s12896-015-0197-x

Table 1.

Purification of recombinant TtAG

Preparation Total protein (mg) Total activity (U) Yield (%) Specific activity (U/mg) Purification fold
Supernatant of crude extract 89.4 2772.2 100.0 31.01 1.0
Supernatant after heating treatment 25.5 3312.0 119.0 129.88 4.2
Affinity (His-Tag column) 8.3 2398.4 86.5 288.96 9.3