. Author manuscript; available in PMC: 2015 Oct 24.

Published in final edited form as: Mod Pathol. 2015 Jul 31;28(10):1390–1399. doi: 10.1038/modpathol.2015.86

Table 2. Specimens with 10% or lower mutant allele frequencies.

Case	Specimens	Tumor %	mut 1	mut 1 (%)	mut 2	mut 2 (%)
1	LN/resection	1–20	G12D/KRAS	3.5
2	Rectum/resection^a	31–50^b	V344M/PIK3CA	8.8^b
33	Colon/resection	31–50^b	Q61H/KRAS	1.95^b
67	Colon/biopsy	1–20	E545K/PIK3CA	7.7	G12D/KRAS	17
75	Rectum/resection^a	1–20	D594G/BRAF	7.2
76	Colon/resection	1–20	Q546H/PIK3CA	4.1	G12D/KRAS	14
91	Colon/resection	41–60^b	E81K/PIK3CA	8.9^b	E545K/PIK3CA	13^b
98	Rectum/resection^a	1–20	G12A/KRAS	9.9
127	Colon/resection	51–70^b	Q61R/NRAS	4.0^b	H1047R/PIK3CA	14^b
144	Colon/biopsy	41–60^b	G15S/KRAS	8.4^b	V600E/BRAF	29
158	Rectum/resection^a	1–20	A59G/KRAS	5.9
174	Colon/biopsy	1–20	E542K/PIK3CA	6.9
202	Colon/resection	61–80^b	G12V/KRAS	5.2^b	G13C/NRAS	36
213	Liver/resection^a	11–30	G12D/KRAS	6.4
217	Rectum/resection^a	1–20	N345K/PIK3CA	6.9
227	Liver biopsy	11–30	V600E/BRAF	7.5
270	Colon/section	11–30	E110del/PIK3CA	9.4	G13D/KRAS	17
282	Colon/resection	61–80^b	E545K/PIK3CA	8.9^b	A146T/KRAS	53
287	Rectum/resection^a	1–20	A146T/KRAS	3.4
302	Rectum/resection^a	31–50^b	E545K/PIK3CA	4.6^b

Abbreviations: mut, mutation; mut 1%, allele frequency of mutation 1; mut 2%, allele frequency of mutation 2; G12A, c.35G4 C; G12D, c.354 G4 A; G12V, C.35G4 T; G13C, c.37G4 T; G13D, c.38G4 A; G15S, c.43G4 A; A59G, c.176C4 G; Q61H, c.183A4 C; Q61R, c.182A4 G; E81K, c.241G4 A; E110del, c.328_330del; A146T, c.436G4 A; V344M, c.1030G4 A; N345K, c.1035T4 A; E542K, c.1624G4 A; E545K, c.1633G4 A; Q546H, c.1638G4 T; D594G, c.1781A4 G; V600E, c.1742A4 G; H1047R, c.3140A4 G

Resection with prior adjuvant therapy.

Mutations with an observed mutant allele frequency at least 10% less than that expected from the estimated tumor cellularity. The expected mutant allele percentage is half of the estimated tumor percentage, assuming heterozygosity with no mutant allele-specific imbalance.