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Acanthamoeba CDC:V621 trophozoites exhibiting varying sizes measuring from 50 to 110 μm. The trophozoites feature single (A–C) or double (D) nuclei or donut‐shaped nucleus (A, B). All trophozoites feature acanthopodia that are characteristically blunt at the tip, contractile vacuole, food vacuole, and a contractile vacuole. The large trophozoite is binucleate, indicating that it has completed karyokinesis and is awaiting cytokinesis. Bar = 10 μm.
