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Phylogram based on 18S rRNA gene sequences from Acanthamoeba Group 1 strains. A representative from Group 2 (i.e., the type strain of A. castellanii) was added as the outgroup. All 10 sequences obtained from CDC:V621 formed a single clade, separate from other Acanthamoeba sequences. The phylogenetic tree was created with the quartet puzzling maximum likelihood method using the program TREE‐PUZZLE. The scale bar estimates evolutionary distance (nucleotide substitutions per position).
