Table 2.
MTB antigens scoring as positive for one or more subjects.
| ORF | subjecta | protein | novel | ||
|---|---|---|---|---|---|
| LTBI US | LTBI India | non-LTBI US | |||
| Rv0007 | 3 | conserved hypothetical membrane protein | yes | ||
| Rv0085 | 2 | hydrogenase C called hypC | yes | ||
| Rv0212 | 3 | asnC-family transcriptional regulator nadR | yes | ||
| Rv0366 | 2 | 4 | hypothetical conserved | yes | |
| Rv0632 | 1 | enoyl-CoA hydratase echA3 | yes | ||
| Rv0798 | 3 | 1 | CFP29 | yes | |
| Rv0984 | 4,5 | pterin-4-alpha-carbinolamine dehydratase moaB2 | yes | ||
| Rv1196 | 1, 2, 3 | 1,2,4,6,8 | PPE18 | ||
| Rv1270 | 3 | lipoprotein LprA | |||
| Rv1387 | 1 | 1,4,5,6,7 | PPE20 | ||
| Rv1411 | 4 | lipoporotein LprG | yes | ||
| Rv1629 | 3 | 3 | DNA polymerase I polA | yes | |
| Rv1728 | 1 | hypothetical conserved | yes | ||
| Rv1837 | 3 | 1 | malate synthase G glcB | yes | |
| Rv1860 | 1 | ala-pro rich secreted | |||
| Rv1886 | 3 | 4 | 4 | ||
| Rv1915 | 1 | isocitrate lyase aceAa | yes | ||
| Rv1926 | 3 | 4 | immunogenic protein mpt63 | yes | |
| Rv1980 | 5 | immunogenic protein mpt64 | |||
| Rv2031 | 2,5 | heat shock protein X, alpha-crystallin homolog | |||
| Rv2151 | 3 | cell division protein ftsQ | yes | ||
| Rv2360 | 5 | hypothetical protein | yes | ||
| Rv2376 | 1 | LMW antigen CFP2 | |||
| Rv2837 | 1 | hypothetical | yes | ||
| Rv2873 | 2 | 1 | lipoprotein MPT83 | ||
| Rv2875 | 1 | immunogenic secreted protein MPT70 | |||
| Rv2885 | 4 | transposase | yes | ||
| Rv3050 | 4 | transcriptional regulator asnC-family | yes | ||
| Rv3191 | 4 | transposase | yes | ||
| Rv3241 | 5 | Conserved protein, similar to many hypothetical proteins and to some putative ribosomal proteins | yes | ||
| Rv3243 | 1 | hypothetical protein | yes | ||
| Rv3247 | 2 | thymidylate synthase | |||
| Rv3319 | 2 | succinate dehydrogenase subunit sdhB | yes | ||
| Rv3345 | 5 | PE-PGRS family protein | yes | ||
| Rv3362 | 1 | ATP/GTP binding protein | yes | ||
| Rv3616 | 1, 2, 3 | 1,4,6 | ala-gly rich protein | ||
| Rv3635 | 1 | 1 | conserved membrane protein | yes | |
| Rv3763 | 1 | 4 | 19 kda lipoprotein antigen precursor lpqH | ||
| Rv3804 | 1, 3 | 1,4 | 4 | secreted fibronectin-binding protein antigen fbpA | |
| Rv3864 | 1 | 4,5,6 | hypothetical protein | ||
| Rv3874 | 3 | 1,2,4,5,6,7,9 | CFP-10, EsxB | ||
| Rv3875 | 1, 2, 3 | 1,4,5 | ESAT-6, EsxA | ||
| Rv3881 | 4,5 | espB, conserved alanine and glycine rich protein | yes | ||
| Rv3920 | 5 | hypothetical protein | yes | ||
a
We enrolled 3 subjects in the US listed as LTBI US 1–3, 2 subjects in the US who were IGRA-negative and at low risk for LTBI, listed as non-LTBI US 4–5, and 9 subjects in India listed as LTBI India 1–9. MTB-specific T-cells were enriched from US subjects using CD137 selection and from India subjects using MTB-IL-2.