Figure 1. Design and fabrication of the multiplexed microfluidic platform.
A. Schematics of the experimental setup. B. Two identical PDMS platforms, each with 10 independent modules, drawn on a circular area representing the size of the silicon wafer. C. A single PDMS platform. D. One of the 10 independent modules of each PDMS platform. E. A zoomed-in area on a module. Each module has 32 rows and 332 columns of replicator units. Neighboring rows are separated by 45 microns, while neighboring columns are separated by 16 microns. F. Design details of a single replicator unit. G. Scanning Electron Microscope (SEM) picture of an area on an independent PDMS module. Scale bar is 100 microns. H. SEM picture of a single replicator unit. Scale bar is 5 microns. I. Time dynamic bright field images showing a trapped mother cell producing a daughter cell. Image sequence starts at top-left and ends at bottom-right.