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. 2015 Oct 20;10(10):e0141131. doi: 10.1371/journal.pone.0141131

Fig 4. The effects of forced weaning on the mRNA expression of Kir2.1 in the mammary gland.

Fig 4

A: mRNA expressions for Kir2.1, LIF, and β-actin in mammary glands. Total RNA was extracted from the abdominal mammary glands of lactating mice and 24-h post-weaning mice. The mRNA expression was examined using conventional RT-PCR. The representative amplifications obtained from three different mice are shown. B: Quantitative analysis of Kir2.1 mRNA expression. The expression levels of Kir2.1 mRNA (relative to β-actin) in lactating and post-weaning mammary glands were examined using real-time PCR. Data represent the mean ± SE (n = 4 for each). *, p < 0.05 vs. lactating glands.