Figure 4.

Preferred amino acid sequence motifs hydrolyzed by SprP. SprP preferentially hydrolyzed tripeptide substrates containing basic (blue) and hydrophobic (black) residues. At amino acid positions 2 and 3, acidic (red) and polar/uncharged residues (green) were also tolerated. Sequence logo was generated as described (Schneider and Stephens 1990; Crooks et al. 2004).