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Promoter activity of sprP in Pseudomonas aeruginosa cultivated at 37°C and 43°C. Pseudomonas aeruginosa harboring plasmid pTZ110 as a control and pTZsprP were cultivated at 37°C (blue) and 43°C (red) and β-galactosidase activity (bars) was determined according to the method of Miller (1972). Bacterial growth curves were determined by measuring the optical density as OD580 nm. Graphs represent average values and error bars indicate standard deviations from triplicate determinations.
