Table 1.
Strains, plasmids, and primers
| Strain or plasmid | Genotype/phenotype | Reference or source |
|---|---|---|
| Strains | ||
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type | Holloway et al. (1979) |
| Escherichia coli | ||
| DH5α | fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ (lacZ)M15 gyrA96 recA1 relA1endA1 thi- 1 hsdR17 | Woodcock et al. (1989) |
| NEB Express Competent | fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS)endA1 Δ(mcrC-mrr)114::IS10 | |
| Plasmids | ||
| pSU-SprPminLS | sprP gene in pSU-HlyA1 | This work |
| pSU-HlyA1 | Apr | Schwarz et al. (2013) |
| pk184-HlyBD | Kmr | Bakkes et al. (2010) |
| pTZsprP | Cbr; sprP-lacZ fusion | Pelzer et al. (2014) |
| Primer for cloning | (5′–3′) | |
| pSUHlyA1_lin_XaHis_for | ATTGATGGCCGTCACCACCACCACCACCACGGAAATTCTCTTGCAAAAAATGTATTA | |
| pSU-HlyA1_lin_rev | CATTTAATTACCTCTTAACCAGTTAATG | |
| SprPminLS_fw | AGAGGTAATTAAATGGCCGAAACACCCCTG | |
| SprP_XaHis_rev | GTGACGGCCATCAATGCGCACGCGCTC | |