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. 2015 Aug 1;4(5):790–802. doi: 10.1002/mbo3.279

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© 2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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UV-visible absorption spectra of various IscR samples in native state. (A) UV-vis spectra of purified IscR (∼60 μmol/L). After Ni-NTA purification, His-tagged IscR was first oxidized (red), then reconstituted in strictly anaerobic conditions (black), and finally oxidized again (Blue). (B) UV-Vis spectra of His-tagged IscR (∼10 μmol/L) purified under reduced conditions (black), iron-limiting conditions (red), or treated with 1 mmol/L H₂O₂ (blue), respectively. (C) UV-Vis spectra of His-tagged IscR (∼10 μmol/L) (black) and its single-site mutants H93A (red), H121A (gray), H127A (purple), C142A (green), P143A (yellow), and the triple-site mutant H93A/H121A/H127A (blue), respectively.