Figure 2.

Metformin Treatment following Injury Rescues Motor Deficits, In Vivo

(A) Experimental paradigm of injury and metformin treatment. Metformin was administered 1 day following injury to lactating mothers.

(B) Mice were tested on their ability to flip from supine position to prone position 1 hr after H/I (n = 11 mice/group, t test).

(C) Latency to fall from the edge of the tube was compared between different treatment groups (n ≥ 6 mice/group, t test).

(D) Expansion of the SE-derived NPC pool in metformin-treated mice (n = 6 mice/group, t test). This metformin-induced expansion of the SE-derived NPC pool was lost in CBP S436A mutants (n ≥ 12 mice/group, t test).

(E) An H/I injury alone expanded the stem cell pool (n = 5 mice/group, two-way ANOVA).

(F) The cylinder-rearing test was used to assess sensory-motor function. Graph depicts non-impaired forepaw preference for mice tested on day 22 (n ≥ 7 mice/group, one-way ANOVA). Met, metformin; CL, contralateral hemisphere to ischemia; IL, ipsilateral hemisphere to ischemia. Data are presented as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01.

See also Figure S1.