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. 2015 Oct 20;11(10):e1005603. doi: 10.1371/journal.pgen.1005603

Fig 4. Promoter analysis of the svh-2 gene.

Fig 4

(A) The svh-2 promoter region. Schematic diagram of the svh-2 transgenes used in rescue experiments are shown. The positions of binding sites for Ets and C/EBP in the svh-2 promoter are indicated by shaded and white boxes, respectively. (B) Identification of the svh-2 promoter region required for axon regeneration. Each construct was transfected into svh-2 mutants and analysed for axon regeneration. The percentages of axons that initiated regeneration 24 hr after laser surgery are shown. Error bars indicate 95% SI. ***P<0.001; NS, not significant. (C) Identification of the svh-2 promoter region required for induction of Psvh-2::nls::venus expression in D-type motor neurons by laser surgery. Each construct was transfected into animals and analysed for svh-2 gene induction in response to laser surgery. The percentages of animals expressing Psvh-2::nls::venus in D-type motor neurons following laser surgery are shown. The numbers (n) of animals examined are shown. Error bars indicate 95% SI. ***P<0.001; NS, not significant.