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. 2015 Oct 20;11(10):e1005603. doi: 10.1371/journal.pgen.1005603

Fig 5. Interaction of ETS-4 with CEBP-1 by yeast two-hybrid assays.

Fig 5

(A) The reporter yeast strain L40u was cotransformed with expression vectors encoding LexA DBD-ETS-4, LexA DBD-ETS-4(S73E), LexA DBD-ETS-4(S73A) and GAL4 AD-CEBP-1 as indicated. Yeasts carrying the indicated plasmids were grown on a selective plate lacking histidine and containing 80 mM 5-aminotriazole for 4 days. (B) The reporter yeast strain NMY51 was cotransformed with expression vectors as indicated. Yeasts carrying the indicated plasmids were grown and β-galactosidase activity was determined in cellular extracts as described in Materials and Methods. Backgrounds were determined by cotransforming cells with the control vector pACTII along with the corresponding pBTM116-ETS-4(WT, S73A or S73E,) and the β-galactosidase activities obtained were subtracted from each set of data. Values are given in Miller units. The data are combined from ten independent experiments and quantified by the Welch’s t test. ***P < 0.001. Error bars represent SEM.