Figure 4. Dose dependent effect of exogenous cFN on TLR4-mediated inflammation in macrophages.

Pooled bone marrow-derived macrophages from EDA^−/−Apoe^−/− and EDA^−/−TLR4^−/−Apoe^−/− mice (n=4-5 mice/group) were stimulated in presence of cFN (0-50 μg/mL) for 24 hours. A. Top panel shows representative immunoblots showing expression of phosphorylated- NFkB p65, total NFkB p65 and β-actin. Bar diagram in middle and bottom panels represent quantification of intensity of phosphorylated- NFkB p65 to total NFkB p65 and phosphorylated- NFkB p65 to β-actin (loading control). N = 3 experiments/group. B&C. ELISA quantification of TNF-α and IL-1β in supernatant medium from cFN treated and untreated macrophages. Data is presented as mean ± SEM. N = 3 experiments/group.