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. 2015 Oct 22;16:833. doi: 10.1186/s12864-015-1962-x

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© Borland et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Evolutionary origin of 49 A. lipoferum 4B HyHKs. The phylogenetic tree showed the maximum likelihood relationships among the 49 transmitter domains (49 sequences, 265 positions). Numbers at branch correspond to SH-like supports calculated with PHYML (for clarity values lesser than 0.90 are not shown). The scale bar indicates the average number of substitution per site. The colored squares indicate the presence of orthologous transmitter (HK) and REC domains in Azospirillum sp. B510 (blue), A. brasilense CBG497 (light green) and A. brasilense Sp245 (dark green). According to phylogenetic analyses, the probable origin of the A. lipoferum 4B HyHK is indicated (red, horizontal gene transfer; purple, gene duplication)