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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Stem Cells. 2015 Feb;33(2):468–478. doi: 10.1002/stem.1851

Figure 3. Inflammatory cytokines released from lungs of CS-exposed mice activated hASC to secrete TSG-6.

Figure 3

(A) Expressions of both murine TNFα and IL1β were up-regulated in the lung after mice were exposed to 3-day CS compared to ambient air control (AC); TNFα was reduced significantly to the level of AC following i.v. injection of hASC; (B) minimum human-specific TSG-6 mRNA was detected in untreated mouse lungs, levels of which was markedly increased 40 hrs following i.v. injection of hASC; (C) In vitro, secretion of TSG-6 protein from hASC is low at baseline but strongly increased after stimulation by human pro-inflammatory cytokines (TNFα and IL1β, but not IFNγ); (D) secretion of TSG-6 from hASC can be activated similarly with cross-species mouse pro-inflammatory cytokines. Mean ± SEM, n = 3; *p < 0.05; **p < 0.01.