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. 2015 Oct 20;10(10):e0141107. doi: 10.1371/journal.pone.0141107

Fig 3. BA-LL37 binding to A. baumannii.

Fig 3

(A) LL-37 binding to A. baumannii cells was determined by flow cytometry. Cells were incubated with different concentrations of BA-LL37 at 4°C in ice-cold PBS overnight. The binding between BA-LL37 and A. baumannii was detected by adding SA-DTAF. The increase of signal was detected and correlated to cells treated with increasing concentrations of LL-37, indicating that LL-37 binds directly to A. baumannii. These data are representative of three independent experiments with similar results. FL1-H indicates the extent of the fluorescence intensity. (B) An immunofluorescence staining assay was performed to verify LL-37 binding to A. baumannii. In the presence of 20 μg/ml LL-37, fluorescence was observed compared to the control (without BA-LL37 treatment). Samples were observed using a Carl Zeiss AXIO IMAGER A1 microscope.