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Total OMPs of A. baumannii were isolated, subjected to 12% SDS-PAGE and detected by western blotting. After electrophoresis, OMPs were stained with Coomassie blue (Lane 1). OMPs in another gel were transferred onto a PVDF membrane using a semi-dry transfer system and blotted with SA-HRP (Lane 2) or an anti-OmpA antibody (Lane 3). The signals were detected using enhanced chemiluminescence (ECL).
