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. 2015 Oct 21;46:122. doi: 10.1186/s13567-015-0252-6

Figure 1.

Figure 1

Effect of free Mmm galactan on secreted embryonic alkaline phosphatase (SEAP) release from HEK-Blue mTLR2 cells ( A ) and HEK-Blue mTLR4 cells ( B ). Null-cells (i.e., HEK-Blue cells neither expressing TLR2 nor TLR4) or TLR-cells were incubated with galactan (EPS) at 0.1, 1 and 10 µg/mL, or with FSL1 lipopeptide and E. coli LPS as positive controls for TLR2 and TLR4 cells respectively. SEAP release after stimulation was measured by a colorimetric method and expressed as mean fold changes (±SD) in comparison to unstimulated cells (n = 3). *p < 0.001 relative to unstimulated controls; **p < 0.05 between different EPS concentrations.