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Effect of free Mmm galactan on recall proliferation of CD4+ T lymphocytes from naïve cattle ( A ) and CBPP-infected cattle ( B ). CFSE-labeled cells were stimulated for 7 days with whole inactivated Mmm (T1/44, 5 μg/mL), galactan (EPS, 10 μg/mL) or PPDa (10 μg/mL). Different symbols represent the net effect of stimulations (i.e., stimulated cultures minus non-stimulated cultures) for each animal individually (n = 3). Asterisks indicate statistically significant differences with PPDa.
