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. 2015 Jul 24;8:312. doi: 10.1186/s13104-015-1260-3

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© Crown. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Western immunoblot and antibody signal ratio of vole brain samples after primary passage of bovine scrapie and comparison with vole-adapted classical scrapie and BSE. WB analysis of PrP^res in voles infected with P75-7 (primary passage) compared with a representative sample of P90-4 (primary passage), vole-adapted classical scrapie and BSE. Replica blots were developed with mAbs SAF84 (upper panel) and 12B2 (lower panel) to compare the antibody reactivity with each sample similar to the discriminative WB used for samples from small ruminants. The graph on the bottom shows the SAF84/12B2 signal ratio relative to the scrapie control. Values higher than 1 denote more C-terminal PK-cleavage and consequential loss of the epitope of 12B2.