Table 3.
Inocula | Post-1990 scrapie pool | P90-1 | Pre-1975 scrapie pool | P75-7 | Bovine C-type BSE | Ovine C-type BSE | H-type BSE | L-type BSE |
---|---|---|---|---|---|---|---|---|
BoPrP-tg110 | ||||||||
1st passage | 643 (1/5) 19 + 21K | 173 ± 3 (6/6) 21K |
457 ± 60 (3/6) 19K | 203 ± 5 (6/6) 19K |
295 ± 12 (6/6) BSE likea | 234 ± 5 (6/6) BSE like | 292 ± 5 (6/6) H-typeb | 207 ± 7 (6/6) L-type |
2nd passage | 282 ± 6 (6/6) 19K |
190 ± 16 (6/6) 21K |
191 ± 4 (6/6) 19K |
200 ± 9 (6/6) 19K |
265 ± 35 (6/6) BSE like | 234 ± 3 (6/6) BSE like | 296 ± 7 (6/6) H-type | 199 ± 1 (6/6) L-type |
PoPrP-tg001 | ||||||||
1st passage | >650 (0/6) | >650 (0/6) | >650 (0/6) | >650 (0/6) | 498 ± 9c (2/12) | 458 ± 11c (15/15) | >650 (0/6)b | >650 (0/6) |
2nd passage | >650 (0/6) | >650 (0/6) | >650 (0/6) | >650 (0/6) | 198 ± 6c (15/15) | 162 ± 4c (13/13) | >650 (0/6)b | >650 (0/6) |
MuPrP-tga20 | ||||||||
1st passage | 571 ± 31 (3/6) 21K |
440 ± 3 (5/6) | 441 ± 67 (6/6) 21K |
480 ± 13 (6/6) | 473 ± 24 (6/6) BSE like | 450 ± 48 (6/6) BSE like | ND | ND |
2nd passage | 159 ± 2 (6/6) 21K |
408 ± 33 (3/6) | 146 ± 41 (3/3) 21K |
ND | 147 ± 3 (6/6) BSE like | 117 ± 3 (6/6) BSE like | ND | ND |
HuPrP-tg340 | ||||||||
1st passage | ND | >650 (0/5) | ND | >650 (0/6) | >700 (1/12) BSE like | 615 ± 84 (4/6) BSE liked | >700 (0/6)b | 629 ± 35 (5/5) L-type |
2nd passage | ND | >650 (0/5) | ND | >650 (0/6) | 690 ± 35 (5/6) BSE like | 564 ± 39 (5/5) BSE like | >700 (0/6)b | 684 ± 45 (4/4) L-type |
OvPrP-tg338 | ||||||||
1st passage | 480 ± 19 (6/6) 21K |
>638 (0/6) | 69 ± 1 (6/6) 21K |
148 ± 2 (5/6) 19K |
704 ± 36 (6/7) BSE likef | 560 ± 60 (5/5) BSE-likef | 595 ± 18 (8/8) H-typeg | 432 ± 19 (6/6) BSE-likef |
2nd passage | ND | 545 (1/5)e 19K | ND | ND | ND | 178 ± 2 (4/4) BSE-likef | 319 ± 10 (6/6) H-typeg | 141 ± 2 (7/7) BSE-likef |
Survival times are displayed in days with standard error of the mean. K denotes the molecular masses (in kDa) of the unglycosylated PrPres band determined by WB.
ND not done.
aInoculum from clinical BSE suspect, 8 year-old Holstein-Friesian cow, UK.
bInoculum from 03-2095, clinically healthy ≥8 year-old cow, France [9, 44].
cPreviously published data from UK and French cases: C-type BSE (BSE2, 8 year-old Hereford crossbred cow, clinical suspect), ovine BSE (pool from 7 ARQ/ARQ sheep intracerebrally infected with brainstem from a naturally affected BSE cow in France) [31].
dBrain pool of ARQ/ARQ sheep inoculated with brain from a naturally infected BSE cow [26].
eOne additional inoculated mouse died at 535 days but no TSE diagnosis was possible.
fPreviously published data from French cases: Ovine BSE (case ARQ1), C-type BSE (case 3), L-type BSE (case 7) [29].
gPreviously published data from French case 2 [30].