Table 3.
Mean survival times and attack rate in transgenic mice inoculated with two ovine scrapie pools, two single case sources of bovine scrapie and single case sources of classical and atypical BSE on primary and second passage
| Inocula | Post-1990 scrapie pool | P90-1 | Pre-1975 scrapie pool | P75-7 | Bovine C-type BSE | Ovine C-type BSE | H-type BSE | L-type BSE |
|---|---|---|---|---|---|---|---|---|
| BoPrP-tg110 | ||||||||
| 1st passage | 643 (1/5) 19 + 21K | 173 ± 3 (6/6) 21K |
457 ± 60 (3/6) 19K | 203 ± 5 (6/6) 19K |
295 ± 12 (6/6) BSE likea | 234 ± 5 (6/6) BSE like | 292 ± 5 (6/6) H-typeb | 207 ± 7 (6/6) L-type |
| 2nd passage | 282 ± 6 (6/6) 19K |
190 ± 16 (6/6) 21K |
191 ± 4 (6/6) 19K |
200 ± 9 (6/6) 19K |
265 ± 35 (6/6) BSE like | 234 ± 3 (6/6) BSE like | 296 ± 7 (6/6) H-type | 199 ± 1 (6/6) L-type |
| PoPrP-tg001 | ||||||||
| 1st passage | >650 (0/6) | >650 (0/6) | >650 (0/6) | >650 (0/6) | 498 ± 9c (2/12) | 458 ± 11c (15/15) | >650 (0/6)b | >650 (0/6) |
| 2nd passage | >650 (0/6) | >650 (0/6) | >650 (0/6) | >650 (0/6) | 198 ± 6c (15/15) | 162 ± 4c (13/13) | >650 (0/6)b | >650 (0/6) |
| MuPrP-tga20 | ||||||||
| 1st passage | 571 ± 31 (3/6) 21K |
440 ± 3 (5/6) | 441 ± 67 (6/6) 21K |
480 ± 13 (6/6) | 473 ± 24 (6/6) BSE like | 450 ± 48 (6/6) BSE like | ND | ND |
| 2nd passage | 159 ± 2 (6/6) 21K |
408 ± 33 (3/6) | 146 ± 41 (3/3) 21K |
ND | 147 ± 3 (6/6) BSE like | 117 ± 3 (6/6) BSE like | ND | ND |
| HuPrP-tg340 | ||||||||
| 1st passage | ND | >650 (0/5) | ND | >650 (0/6) | >700 (1/12) BSE like | 615 ± 84 (4/6) BSE liked | >700 (0/6)b | 629 ± 35 (5/5) L-type |
| 2nd passage | ND | >650 (0/5) | ND | >650 (0/6) | 690 ± 35 (5/6) BSE like | 564 ± 39 (5/5) BSE like | >700 (0/6)b | 684 ± 45 (4/4) L-type |
| OvPrP-tg338 | ||||||||
| 1st passage | 480 ± 19 (6/6) 21K |
>638 (0/6) | 69 ± 1 (6/6) 21K |
148 ± 2 (5/6) 19K |
704 ± 36 (6/7) BSE likef | 560 ± 60 (5/5) BSE-likef | 595 ± 18 (8/8) H-typeg | 432 ± 19 (6/6) BSE-likef |
| 2nd passage | ND | 545 (1/5)e 19K | ND | ND | ND | 178 ± 2 (4/4) BSE-likef | 319 ± 10 (6/6) H-typeg | 141 ± 2 (7/7) BSE-likef |
Survival times are displayed in days with standard error of the mean. K denotes the molecular masses (in kDa) of the unglycosylated PrPres band determined by WB.
ND not done.
aInoculum from clinical BSE suspect, 8 year-old Holstein-Friesian cow, UK.
bInoculum from 03-2095, clinically healthy ≥8 year-old cow, France [9, 44].
cPreviously published data from UK and French cases: C-type BSE (BSE2, 8 year-old Hereford crossbred cow, clinical suspect), ovine BSE (pool from 7 ARQ/ARQ sheep intracerebrally infected with brainstem from a naturally affected BSE cow in France) [31].
dBrain pool of ARQ/ARQ sheep inoculated with brain from a naturally infected BSE cow [26].
eOne additional inoculated mouse died at 535 days but no TSE diagnosis was possible.
fPreviously published data from French cases: Ovine BSE (case ARQ1), C-type BSE (case 3), L-type BSE (case 7) [29].
gPreviously published data from French case 2 [30].