Fig. 2.

Effect of lunasin-like polypeptides on the Caco-2 cells proliferation. Box-plot showing aggregate data for human colon adenocarcinoma (Caco-2) cells proliferation after treatments of 24 (panel a), 48 (panel b), and 72 h (panel c) with water/salt soluble extracts obtained from control doughs (D), without bacterial inoculum, and sourdoughs (S), started with selected lactic acid bacteria, made with Italian legume flours. Protein concentration of 0.1, 1.0, and 10 mg/ml were assayed. Data were expressed as the mean percentage of viable cells compared to the control culture, grown in basal media without the addition of the WSE. The centre line of the box represents the median (open square), the top and bottom of the box represent the 75th and 25th percentile of the data, respectively. The top and bottom of the bars represent the 5th and 95th percentile of the data, respectively. Outliers (open circle) and extremes (aterisk) are represented