Table 1.
Primers for new markers successfully mapped near the Qcrs.cpi-3B locus#
| Marker | Forward primer/position (bp) | Reverse primer/position (bp) | Product size (bp) |
|---|---|---|---|
| CS3BLCR-01 | AAACAGAGTAACAGACCGACC/773070474 | TTCAGATAAGGAAACCAAACA/773070830 | 357 |
| CS3BLCR-02 | CATACATACTCCCTCTGTTACCT/773095727 | CTTCCCTTTGCTGCGTGT/773096117 | 391 |
| CS3BLCR-03 | GGTCTCTTCGCTGACAAA/750842960 | CCTTGCTTGCTTATGTGCT/750843318 | 359 |
| CS3BLCR-04 | GCTTTGAGTTATGTATCCGTG/770066939 | TAAGAAACCAGCGTCAGAAA/770067148 | 210 |
| CS3BLCR-05 | ATCTGGGATAATGAGGTGG/770736229 | GAAGACCAACATAGTAATCGG/770736554 | 326 |
| CS3BLCR-06 | TCCTTATTCCTCTTCCCTAAA/770210969 | TTGACTTGTTTCTTATGTCCTG/770211217 | 249 |
| CS3BLCR-07 | GAGGGAGGTCTGGTTGGG/770225517 | AAGCGGAGTGTTGTGTTG/770225671 | 155 |
| CS3BLCR-08 | TGATTACAACAAACAAACCC/770294691 | ATACACTGAACAAACGGATAA/770294978 | 288 |
| CS3BLCR-09 | GTAGAAATAGGGACAACGGAG/770304690 | ACAAGAAACGGCAGATGA/770305044 | 355 |
| CS3BLCR-10 | AAAGCCACCTAATGAAGAAAC/770331178 | GAAGAAGAAGAGGAGCGG/770331485 | 308 |
| CS3BLCR-11 | TGTTGTTTGGGTAGTCATTCT/770349503 | GCTCCAGTGCTTGTAGTCA/770349800 | 298 |
| CS3BLCR-12 | AAGAAGAGGAGCAGCAAAG/770552012 | AAAGCCACCTAATGAAGAAAC/770552259 | 248 |
| CS3BLCR-13 | ATGGAGGAGACGAAACTCA/770843419 | GTTATCTTGGTATGCGGGT/770843678 | 260 |
#Primer positions and the product sizes were based on those reported in the chromosome 3B pseudomolecule; the annealing temperature used for all of the primer sets was 55 °C