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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: J Immunol Methods. 2011 Aug 11;373(1-2):1–7. doi: 10.1016/j.jim.2011.08.002

Figure 1. Gating strategy for flow cytometry identification of iNKT cells among blood mononuclear cells.

Figure 1

Lower panels: PBS57/CD1d-tetramer staining on (A) CD3positive cells or (B) CD19positive cells. Note the significant background PBS57/CD1d-tetramer staining on (CD19-expressing) B cells warranting use of an anti-CD19 antibody as a dump channel.