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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: J Immunol Methods. 2011 Aug 11;373(1-2):1–7. doi: 10.1016/j.jim.2011.08.002

Figure 3. Characterization of purified human iNKT cells.

Figure 3

(A) Positive staining of CD1d tetramer-purified iNKT-cell with the 6B11 antibody. (B) iNKT cell proliferation when stimulated with plate-bound anti-CD3 (OKT3) and soluble anti-CD28 antibodies (as described in Ladd et al., 2010). (C) Cytokine profile of purified iNKT cells reconstituted (1 iNKT:1000 mononuclear cells) with CD1d-tetramer-depleted (FACS) autologous cord blood (CB) or adult peripheral blood (APB) mononuclear cells following stimulation with α-galactosylceramide (100 ng/mL; 96 hours). Cytokine levels are presented (mean, error bars: standard deviation of duplicate ELISA analyses) after subtraction of unstimulated cells.