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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: J Immunol Methods. 2011 Aug 11;373(1-2):1–7. doi: 10.1016/j.jim.2011.08.002

Table 1.

Typical number of iNKT cells extracted from adult or neonatal (cord) blood.

Adult Blood (n = 5) Adult Buffy Coat (n = 3) Cord Blood (n = 15)
Starting Volume (mL) 150–200 50 50–150
Mononuclear cells/mL of blood 1–2 million/mL 20–25 million/mL 4–6 million/mL
iNKT cells/CD3+ cells (%) 0.001–0.1% 0.001–0.1% 0.05–0.3%
Percentage iNKT cells after purification >95% >95% >95%
Number of iNKT cells extracted 3,000–50,000 7,000–100,000 80,000–250,000