Figure 5. SECE preferably labels MNP progenitors with administration of minimal dosage of tamoxifen.
(A-C) LacZ reporter expression in SECE; R26R+/- embryos when tamoxifen is administered at 10 μg/g body weight by I.P. injection at E7.5. (A) In E11.5 embryos, reporter expression is detected in the neural cranium (black arrow), the mandible (blank arrow) and the medial nasal process (red arrow). (B) At E10.5, LacZ reporter is expressed in the neural cranium (black arrow), the mandible (blank arrow) and the medial nasal process (red arrow). (B′) Magnification of the craniofacial region defined in B. (C) E9.5 embryo expresses LacZ in progenitors of the neural cranium (black arrow), the mandible (blank arrow) and cells beneath the developing retina (red arrow). (C′) Magnification of craniofacial region defined in C.