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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Dyn. 2015 Sep 2;244(11):1439–1455. doi: 10.1002/dvdy.24322

Figure 3.

Figure 3

Retinal progenitor proliferation in wild-type and clo mutant embryos. A-D. Immunofluorescence images of wild type (A,C) and clo−/− (B,D) retinas stained with anti-phosphohistone 3 (PH3) and counterstained with DAPI (blue); samples obtained at 30 hpf (A,B) and 54 hpf (C,D). E-G. Numbers of PH3+ nuclei/section are not significantly different in clo−/− vs. wild-type retinas at 30 hpf (E), but are significantly different at 54 hpf (F; p<0.001); these differences are most evident in the outer nuclear layer (G; ONL; p<0.001), but not in the inner nuclear layer (INL) or circumferential germinal zone (CGZ). H. Mitotic index (# PH3+ nuclei/total number of retinal cells per section) is not significantly different in clo−/− as compared to wild-type. Scale bars = 50 μm (A applies to A,B; C applies to C,D).