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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Dyn. 2015 Sep 2;244(11):1404–1414. doi: 10.1002/dvdy.24321

Figure 2. Chemical inhibition of histone deacetylases decreases prostatic bud number and alters branching morphogenesis in mouse UGS explant culture.

Figure 2

14 dpc male mouse UGS explants were grown for 7 days in media containing androgen (10nM, dihydrotestosterone, DHT) and vehicle alone (0.1%DMSO) or vehicle containing trichostatin A (10–100nM TSA). (A) In situ hybridization was used to visualize NK3 transcription factor locus 1 (Nkx3-1, purple), which marks prostatic bud epithelium and immunohistochemistry (IHC) was used to visualize E-cadherin (orange), which marks UGS epithelium. (B) Prostatic bud number and (C) buds undergoing branching morphogenesis and (D) number of tips per bud undergoing branching morphogenesis were quantified from n=5 UGSs per group. Results are mean ± SEM. Asterisk indicates significant differences (p ≤ 0.05) from control, arrowheads and insets indicate representative buds undergoing branching morphogenesis.