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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dev Dyn. 2015 Sep 2;244(11):1404–1414. doi: 10.1002/dvdy.24321

Figure 3. Chemical inhibition of histone deacetylases in mouse UGS explant culture increases Bmp2 and 4 transcript abundance and enhances Bmp2 histone acetylation.

Figure 3

14 dpc male mouse UGS explants were grown for 7 days in media containing androgen (10nM, dihydrotestosterone, DHT) and vehicle alone (0.1%DMSO) or vehicle containing trichostatin A (100nM TSA). (A–D) Real time quantitative PCR (QPCR) was used to determine relative transcript abundance of Bmp2, Bmp4, Bmp6 and Bmp7. (E–F) Chromatin immunoprecipitation (ChIP) was used to determine histone H3 lysine 27 acetylation (H3K27ac) at the Bmp2 and Bmp4 promoter region. Results are mean ± SEM. Asterisk indicates significant differences from control p ≤ 0.05, n=5 per group.