Figure 6. Inhibition of BMP signaling partially restores prostatic branching morphogenesis in UGS explants treated with a histone deacetylase inhibitor.

14 dpc male mouse UGS explants were grown for 7 days in media containing androgen (10nM, dihydrotestosterone, DHT) and trichostatin A (100nM, TSA), recombinant NOGGIN (5μg/ml) or both. (A) Explants were stained as described in Fig. 2 to visualize prostatic buds. (B) The number of tips per branching bud was determined from the stained explants. Results are mean ± SEM. Asterisk indicates significant differences from control p ≤ 0.05, n=5 per group; arrowheads and insets indicate representative buds undergoing branching morphogenesis.