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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Virology. 2015 Sep 2;485:371–382. doi: 10.1016/j.virol.2015.08.011

Figure 1.

Figure 1

Structure of DENV DIII and library design. (A) Prefusion structure of homodimeric E, with the three domains on one of the subunits colored (DENV-2 shown as an example, from PDB code 1OK3, ref. 58). (B) View of the DENV-2 DIII as an example down the 4E11 structural epitope. The A- and G-strands are labeled in the inset, and the locations of the residues included in the combinatorial alanine scanning mutagenesis analysis are shown on the larger graphic, which has the same orientation as the inset. Position 360 was included in the analysis for DENV-2 only. (C) Overlay of the four DENV DIII domains bound to the 4E11 scFv (PDB codes 3UZQ, 3UZV, 3UZE, and 3UYP, ref. 18). (D) Amino acid alignment of the four DENV DIII domains. Residues that are absolutely conserved are colored red, those that are conserved in three of the serotypes are colored blue. The location of the β-strands is indicated with arrows; the residues subjected to combinatorial scanning mutagenesis are indicated with asterisks except for position 360 (^), which was analyzed in DENV-2 only.