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Validation of combinatorial alanine scanning results for DENV-1 (A) and DENV-2 (B) point mutants by competition phage ELISA. (A) Competition of phage expressing WT, E309A, and V312A DENV-1 DIII for binding to immobilized 4E11 by soluble WT DENV-1 DIII protein. (B) Similar analysis for WT, L387A, and W391A DENV-2 DIII expressing phage for competition against soluble WT DENV-2 DIII protein.
